Effect of miR-221-specific inhibitor on the proliferation and apoptosis of human colorectal carcinoma cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 674-677, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-332577
ABSTRACT
<p><b>OBJECTIVE</b>To investigate miRNA-221 expression in human colorectal carcinoma (CRC) cells and the effects of miR-221-specific inhibitor on the proliferation and apoptosis of CRC cells.</p><p><b>METHODS</b>Four human CRC cell lines (HT-29, Lovo, SW-480, and CaCO2) were examined for miRNA-221 expression using real-time Q-PCR. The specific 2,-methoxy-modified RNA oligonucleotides of miR-221 (anti-miR-221) were synthesized and transfected into Caco2 cells via liposome, and the changes in the expression of miR-221 in the cells were detected by real-time Q-PCR. The proliferation and apoptosis of the transfected CRC cells were detected using MTT assay and flow cytometry.</p><p><b>RESULTS</b>The 4 human CRC cells showed significantly upregulated expression of miR-221 compare with HUVECs (P<0.01). The miR-221-specific inhibitor, anti-miR-221, significantly inhibited the expression of miR-221 in Caco2 cells and suppressed the cell proliferation, causing also obvious cell apoptosis (P<0.01).</p><p><b>CONCLUSION</b>The miR-221-specific inhibitor shows potent inhibitory effect on the growth of CRC cells, suggesting its value as a potential anti-tumor candidate for treatment of CRC.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Neoplasias Colorrectales
/
Apoptosis
/
MicroARNs
/
Línea Celular Tumoral
/
Proliferación Celular
/
Metabolismo
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2011
Tipo del documento:
Artículo
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