Application of Multiplex PCR for the Screening Di(b-),Co(a-) Rare Blood Group in Chinese Xinjiang Minorities / 中国实验血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the distribution of Colton and Diego rare blood group antigens of blood donors in Chinese Xinjiang minorities.</p><p><b>METHODS</b>A multiplex PCR was applied to screen for alleles antigens Diand Coin 1020 randomly selected healthy donors of Chinese Xinjiang minorities by using each 5 samples mixed detection method. The samples in the positive pools were further tested individually. Furthermore, the positive samples, including Di/Diand Co/ Cogenotypes were tested via 2 PCR-SSP assays for high frequency allele Diand Coto get the rare genotypes Di, Co.</p><p><b>RESULTS</b>Among 1020 samples 12 cases with Coallele, 45 cases with Diand 1 case with Diwere identified.</p><p><b>CONCLUSION</b>The frequencies of Diand Coalleles are 2.30% and 0.59%, respectively. The information of rare blood donors obtained from the screening can provide a reference for matched blood transfusion, and further enrich the National Rare Blood Bank of China.</p>