Influence of TIEG1 on apoptosis of HL-60 cells and expression of Bcl-2/Bax / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 587-590, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-332731
ABSTRACT
This study was aimed to investigate the influence of TIEG1 on apoptosis of HL-60 cells and the expression of Bcl-2/Bax. Different concentration of TIEG1 were used to treat HL-60 cells, the cell growth inhibition rate was detected by MTT method. After treating HL-60 cells with 12.03 ng/ml TIEG1, cell apoptosis was detected with flow cytometry. Bcl-2 and Bax was detected with RT-PCR. The results showed that TIEG1 had inhibitory effect on HL-60 cell proliferation, and in time-and dose-dependent manners. The more obvious inhibitory effect was observed in HL-60 cells treated with TIEG1 of 12.03 ng/ml. During the course of cell apoptosis, Bax expression increased, but Bcl-2 expression decreased (P < 0.05). It is concluded that TIEG1 inhibits HL-60 cell proliferation and induces apoptosis in time and dose-dependent manners. During the course of HL-60 cells apoptosis induced by TIEG1, Bcl-2/Bax are associated with HL-60 cell apoptosis induced by TIEG1.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Regulación Leucémica de la Expresión Génica
/
Apoptosis
/
Células HL-60
/
Proteínas Proto-Oncogénicas c-bcl-2
/
Proliferación Celular
/
Proteína X Asociada a bcl-2
/
Factores de Transcripción de la Respuesta de Crecimiento Precoz
/
Factores de Transcripción de Tipo Kruppel
/
Metabolismo
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2013
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS