Changes of neuronal calcium channel following brain damage induced by injection of pertussis bacilli in rats / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 224-227, 2002.
Artículo
en Inglés
| WPRIM
| ID: wpr-332963
ABSTRACT
<p><b>OBJECTIVE</b>To explore changes of neuronal calcium channel following brain damage induced by injection of pertussis bacilli in rats, and to investigate the relationship between cytosolic free calcium concentration ([Ca(2+)](i)) in the synaptosome and Ca(2+)-ATPase activities of mitochondria.</p><p><b>METHODS</b>The level of [Ca(2+)](i) in the synaptosome and Ca(2+)-ATPase activities of mitochondria in the acute brain damage induced by injection of pertussis bacilli (PB) in rat was determined and nimodipine was administrated to show its effects on [Ca(2+)](i) in the synaptosome and on alteration of Ca(2+)-ATPase activity in the mitochondria. Seventy-three rats were randomly divided into four groups, ie, normal control group (Group A), sham-operation control group (Group B), PB group (Group C) and nimodipine treatment group (Group D).</p><p><b>RESULTS</b>The level of [Ca(2+)](i) was significantly increased in the PB-injected cerebral hemisphere in the Group C as compared with that in the Group A and the Group B at 30 minutes after injection of PB. The level of [Ca(2+)](i) was kept higher in the 4 hours and 24 hours subgroups after the injection in the Group C (P<0.05). In contrast, the Ca(2+)-ATPase activities were decreased remarkably among all of the subgroups in the Group C. Nimodipine, which was administered after injection of PB, could significantly decrease the [Ca(2+)](i) and increase the activity of Ca(2+)-ATPase (P<0.05).</p><p><b>CONCLUSIONS</b>The neuronal calcium channel is opened after injection of PB. There is a negative correlation between activities of Ca(2+)-ATPase and [Ca(2+)](i). Nimodipine can reduce brain damage through stimulating the activities of Ca(2+)-ATPase in the mitochondria, and decrease the level of [Ca(2+)](i) in the synaptosome. Treatment with nimodipine dramatically reduces the effects of brain damage induced by injection of PB.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Sinaptosomas
/
Bordetella pertussis
/
Lesiones Encefálicas
/
Bloqueadores de los Canales de Calcio
/
Nimodipina
/
Calcio
/
Análisis de Varianza
/
Ratas Sprague-Dawley
/
ATPasas Transportadoras de Calcio
Límite:
Animales
Idioma:
Inglés
Revista:
Chinese Journal of Traumatology
Año:
2002
Tipo del documento:
Artículo
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