Molecular biological survey of tick-born arboviruses in southern part of Xinjiang / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>BACKGROUND</b>To disclose the species and distribution of tick-borne arboviruses in the southern part of Xinjiang.</p><p><b>METHOD</b>Totally 5045 ticks were collected from 36 collecting sites of 23 places in the southern Xinjiang, which were made into cDNA pools with pd(N)6 primer through RT-PCR method. Then PCR was used to detect viral nucleotide sequence from cDNA.</p><p><b>RESULTS</b>All 34 cDNAs showed negative to flavivirus and California serogroup virus primers; but nairovirus and primers derived from Xinjiang hemorrhagic fever virus had amplified and yielded some obvious bands corresponding to the nucleotide sequences of Xinjiang hemorrhagic fever virus. A phylogenetic analysis was done to the obtained partial sequences of L and S segments.</p><p><b>CONCLUSION</b>Nucleotide sequences of Neither flaviviruses nor California serogroup viruses were detected from the samples. However partial L segment sequence was first reported in China. Phylogenetic analysis of partial L and S segments disclosed the molecular characteristic of Xinjiang hemorrhagic fever virus.</p>