Inoculation of plasmid expressing the dengue 2 NS1 gene elicits immunity in mice / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 71-73, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-333045
ABSTRACT
<p><b>OBJECTIVE</b>To study cellular and humoral immune responses to NS1 protein in mice inoculated intramuscularly with recombinant plasmid expressing dengue 2 NS1 gene.</p><p><b>METHODS</b>The eukaryotic expressing plasmid pCNX2-NS1 was injected into tibialis anterior muscle in mice. The mice were subsequently boosted with the same dose and same method twice after the initial inoculation. The mice were killed at four-week intervals and their serum and spleen cells were harvested for further test.</p><p><b>RESULTS</b>Dengue 2 antibodies were detectable in the sera from inoculated animals four weeks after the last boost. The changes of CD4+ T lymphocyte and CD8+ T lymphocyte were also determined by flow cytometry.</p><p><b>CONCLUSION</b>The recombinant plasmid containing dengue 2 NS1 genes is immunogenic in intramuscularly inoculated mice. The vaccinated mice produced dengue-2 specific and long lasting immunity.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plásmidos
/
Virología
/
Sangre
/
Linfocitos T CD4-Positivos
/
Expresión Génica
/
Inmunización
/
Proteínas no Estructurales Virales
/
Vacunas Combinadas
/
Linfocitos T CD8-positivos
/
Vacunas de ADN
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2005
Tipo del documento:
Artículo
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