Determination of SARS-CoV by simplified nested fluorescent RT-PCR / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 176-178, 2005.
Article
en Zh
| WPRIM
| ID: wpr-333069
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a simple rapid and sensitive nested RT-PCR method for detection of SARS coronavirus RNA by designing the specific primers for SARS and optimizing the parameters for PCR.</p><p><b>METHODS</b>Primers and fluorescent probes were designed according to the sequences of SARS coronavirus genes available from GenBank. The optimization of the parameters for PCR was performed in PE 7700 thermal cycle. The 36 serum samples and 40 mouthwash of SARS patients and 80 samples of healthy people were tested.</p><p><b>RESULTS</b>The positive rate of patient serum and mouthwash was 33.6%, (12/36) and 67.5%, (27/40), respectively, while the positive rate of healthy people was zero (0/160).</p><p><b>CONCLUSION</b>The simple nested RT-PCR method was a rapid, efficient and sensitive one for SARS early diagnosis.</p>
Texto completo:
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Índice:
WPRIM
Asunto principal:
Virología
/
Sangre
/
ARN Viral
/
Reproducibilidad de los Resultados
/
Sensibilidad y Especificidad
/
Cartilla de ADN
/
Secreciones Corporales
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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Síndrome Respiratorio Agudo Grave
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Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2005
Tipo del documento:
Article