In vitro infection of human hepatoma (Hep G2) cell line by hepatitis B virus positive serum / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 169-171, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-333071
ABSTRACT
<p><b>OBJECTIVE</b>To establish a culture system of HBV positive serum infected Hep G2 cells in vitro.</p><p><b>METHODS</b>Hep G2 cells were seeded into six-well cluster dishes, at 1 x 10(-6) cells per well and incubated with 3 ml 10% fetal calf serum/ Dulbecco's modified Eagle's medium (10% FCS/DMEM) at 37 degrees in 5% CO2 air. At 24 h after plating, infection group Hep G2 cells were cultured with 0.5 ml HBV positive serum, in control group HBV negative serum was used, 24 h later the inoculums was removed. The cells were then extensively washed with 0.01 mol/L phosphate-buffered saline (PBS). After washing with PBS, 4 ml 2% FCS/DMEM were added to each well and the medium was collected every 12 h. ELISA method was used to detect HBsAg in culture medium. HBV DNA in cells and culture medium was detected by PCR.</p><p><b>RESULTS</b>In infection group, HBsAg could be detected from cell culture medium from 12 h (after PBS washed) to 84 h. HBV DNA could be detected by PCR in culture medium and cells.</p><p><b>CONCLUSION</b>Infection of Hep G2 cells by HBV positive serum is feasible.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Virología
/
Sangre
/
ADN Viral
/
Ensayo de Inmunoadsorción Enzimática
/
Virus de la Hepatitis B
/
Reacción en Cadena de la Polimerasa
/
Carcinoma Hepatocelular
/
Línea Celular Tumoral
/
Suero
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2005
Tipo del documento:
Artículo
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