In vitro study on Hep G2 cell infected by hepatitis C virus / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 156-158, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-333075
ABSTRACT
<p><b>OBJECTIVE</b>To establish hepatitis C virus (HCV) infected cell model which is similar to the infection in vivo and can support HCV to replicate for a long time.</p><p><b>METHODS</b>After infected with HCV-positive serum, Hep G2 cells were cultured for 60 days. Nested RT-PCR was used to detect plus and minus HCV RNA in cultured cells and supernatants.</p><p><b>RESULTS</b>Plus HCV RNA was detected intermittently in Hep G2 cells during 2-30 days, minus HCV RNA was detected during 3-30 days after infection, the detection rate was similar to plus HCV RNA. Plus and minus HCV RNA can be still intermittently detected during 31-60 days after infection. However, the detection rate gradually declined. Plus HCV RNA was also found intermittently positive in the supernatant, and the detection rate was consistent to that in cells. Minus HCV RNA was not detected in the supernatant.</p><p><b>CONCLUSION</b>Hep G2 cells were susceptible to HCV, and could support HCV to replicate for a relatively long time. Hep G2 is an ideal HCV infection cell model.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Replicación Viral
/
ARN Viral
/
Hepacivirus
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Línea Celular Tumoral
/
Genética
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2005
Tipo del documento:
Artículo
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