Study on promoter methylation status of E-cadherin gene in nasopharyngeal carcinoma cell lines / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 532-536, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-333258
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of methylation on E-cadherin inactivation in nasopharyngeal carcinoma (NPC) cell line HNE1 and CNE2, as well as evaluate the inhibitory effect of 5-aza-2'-deoxycytidine (5-Aza-dC) on cell abilities of proliferation and invasion.</p><p><b>METHODS</b>The expression level of E-cadherin was measured by RT-PCR, Western blot and immunohistochemistry (polymer method), the methyaltion status was analyzed by methylation-specific PCR (MSP), and cell proliferation and invasion were examined by MTT and invasion assay, separately before and after treatment with demethylating agent 5-Aza-dC.</p><p><b>RESULTS</b>The expression level of E-cadherin was down-regulated compared with the normal tissue, simultaneously partially methylated in gene promoter. Treatment with 20 µmol/L 5-Aza-dC increased the expression of E-cadherin and reduced the methylation degree. Moreover, it also significantly suppressed cell growth (27.6% for HNE1 cells and 34.3% for CNE2 cells, P < 0.05) and invasiveness (37.2% for HNE1 cells and 29.7% for CNE2 cells, P < 0.05).</p><p><b>CONCLUSIONS</b>Aberrant methylation around gene promoter region may play an important part in down regulation of E-cadherin in NPC, suggesting a potential therapeutic strategy for demethylating agents such as 5-Aza-dC.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Azacitidina
/
Cadherinas
/
Regulación hacia Abajo
/
Regulación Neoplásica de la Expresión Génica
/
Movimiento Celular
/
Neoplasias Nasofaríngeas
/
Regiones Promotoras Genéticas
/
Metilación de ADN
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Pathology
Año:
2010
Tipo del documento:
Artículo
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