Inhibition of proliferation of salivary adenoid cystic carcinoma by targeting gene transfer / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the expression of gene TRAIL driven by human telomerase reverse transcriptase (hTERT) promoter in SACC-83 cell tumor necrosis factor related apoptosis in dncing ligand.</p><p><b>METHODS</b>After pACTERT-TRAIL plasmid transfected was into SACC-83 and HEL cells through liposome, the expression of TRAIL was examined using RT-PCR technique, the cells' survival rate by methyl thiazolyl tetrazolium (MTT) method and apoptosis rate by flow cytometry.</p><p><b>RESULTS</b>Expression of extrinsic TRAIL gene driven by hTERT promoter was detected in SACC-83 cells, and not detected in human embryonic lung fibroblast (HEL) cells. After transfection of pACTERT-TRAIL, the proliferation of SACC-83 cells was significantly inhibited, and its apoptotic rate was promoted, whereas no inhibited effect was observed on HEL cells and its apoptotic rate showed little change.</p><p><b>CONCLUSIONS</b>hTERT promoter can be used to induce tumor-specific expression of TRAIL gene and apoptosis in SACC-83 cells.</p>