Methylglyoxal inhibits human umbilical vein cell migration in vitro by down-regulating integrinβ3 / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To explore the effects of methylglyoxal on endothelia cell migration.</p><p><b>METHODS</b>Human umbilical vein endothelial cells (HUVECs) were stimulated by serial concentrations of methylglyoxal (MGO, 0, 25, 50, 100 and 200 µmol/L) for 24 h, and the cell migration was assessed by scratch wound and Transwell assay. The expression of integrin β3 in the treated cells was examined by immunoblotting, and the effect of an anti-β3 antibody, LM609, on cell migration was investigated.</p><p><b>RESULTS</b>Methylglyoxal significantly inhibited HUVEC migration in a concentration-dependent manner (P<0.05). Methylglyoxal decreased the expression of integrin β3 in a time- and concentration-dependent manner (P<0.05). LM609 also significantly inhibited HUVEC migration (P<0.05).</p><p><b>CONCLUSION</b>Methylglyoxal inhibits HUVEC migration in vitro by down-regulating integrin β3 expression.</p>