Effect of cancer-associated fibroblasts on proliferation and invasion of gallbladder carcinoma cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1149-1154, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-333666
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of cancer-associated fibroblasts (CAFs) on the growth and invasion of gallbladder cancer cells.</p><p><b>METHODS</b>The CAFs were isolated from human primary gallbladder carcinoma tissues by tissue culture and digestion methods. The cells were purified by differential adhesion method, and the primary cells were identified morphologically and immunocytochemically. The proliferation and invasion of two human gallbladder carcinoma cell lines (SBC-996 and GBC-SD) co-cultured with CAFs were detected by MTT and Transwell chamber assays.</p><p><b>RESULTS</b>Gallbladder carcinoma CAFs were isolated successfully by both tissue culture and enzyme digestion methods, and the latter method was more convenient and efficient. MTT and Transwell assays showed that CAFs significantly promoted the proliferation and invasion of the two gallbladder carcinoma cell lines.</p><p><b>CONCLUSION</b>CAFs can promote the proliferation and invasion of gallbladder carcinoma cells in vitro, suggesting the important role of CAFs in the development of gallbladder carcinoma.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Carcinoma
/
Técnicas de Cocultivo
/
Biología Celular
/
Línea Celular Tumoral
/
Proliferación Celular
/
Fibroblastos
/
Neoplasias de la Vesícula Biliar
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2015
Tipo del documento:
Artículo
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