Your browser doesn't support javascript.
loading
Lentivirus-mediated shRNA targeting ZNF217 suppresses cell growth, migration, and invasion of glioma cells in vitro / 南方医科大学学报
Journal of Southern Medical University ; (12): 1024-1033, 2015.
Artículo en Chino | WPRIM | ID: wpr-333691
ABSTRACT
<p><b>OBJECTIVE</b>To explore the role of ZNF217 in regulating cell proliferation, migration and invasion in glioma cells.</p><p><b>METHDOS</b>A lentivirus-mediated shRNA-ZNF217 vector was infected into glioma U251 cells, and the interference efficiency was examined by Western blotting. MTT assay, flow cytometry, Transwell assay, and Boyden chamber assay were used to analyze the changes in cell proliferation, migration and invasion. Western blotting was used to detect the changes in ZNF217-related genes in the cells.</p><p><b>RESULTS</b>shRNA-ZNF217 transfection significantly inhibited the expression of ZNF217 in U251 cells and suppressed the cell migration, invasion, growth, and cell cycle transition. ZNF217 knockdown downregulated the expression of pPI3, pAKT, C-Myc, and the mesenchyme biomarker N-cadherin, and stimulated the expression of the epithelium biomarker E-cadherin.</p><p><b>CONCLUSION</b>ZNF217 promotes cell migration, invasion, and growth by activating PI3K/AKT signal to upregulate C-Myc and by modulating the genes associated with epithelial-mesenchymal transition in glioma cells.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / ARN Mensajero / Transfección / Cadherinas / Ciclo Celular / Transactivadores / Movimiento Celular / Lentivirus / ARN Interferente Pequeño / Línea Celular Tumoral Límite: Humanos Idioma: Chino Revista: Journal of Southern Medical University Año: 2015 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / ARN Mensajero / Transfección / Cadherinas / Ciclo Celular / Transactivadores / Movimiento Celular / Lentivirus / ARN Interferente Pequeño / Línea Celular Tumoral Límite: Humanos Idioma: Chino Revista: Journal of Southern Medical University Año: 2015 Tipo del documento: Artículo