Metabolic footprint in conditioned culture medium of placental explants: a comparison between early-onset and late-onset severe preeclampsia / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1547-1550, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-333867
ABSTRACT
<p><b>OBJECTIVE</b>To explore the differences of metabolic footprint in the conditioned culture medium of placental explants between early-onset and late-onset severe preeclampsia.</p><p><b>METHODS</b>In 13 cases of early-onset severe preeclampsia and 14 cases of late-onset severe preeclampsia, the placentas were sampled at the surface of the maternal placenta. High performance liquid chromatography-mass spectrometry (HPLC-MS) was used to determine the differences in the metabolites in the conditioned culture medium of the placental villous explants cultured in 6% atmospheric O(2) for 96 h. Standard samples were used to establish the tryptophan and kynurenine chromatography library by HPLC-MS to analyze the concentration of tryptophan and kynurenine in the conditioned culture medium.</p><p><b>RESULTS</b>Thirty-six metabolites showed statistically significant differences between early-onset and late-onset severe preeclampsia (P<0.05). The concentration of kynurenine was significantly higher in early-onset severe preeclampsia than in late-onset severe preeclampsia (P<0.05).</p><p><b>CONCLUSION</b>Early-onset and late-onset severe preeclampsia may have different pathogeneses. By detecting the concentration of metabolites, metabolomic strategies provide a new means for predicting the onset time of severe preeclampsia.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Ornitina
/
Placenta
/
Preeclampsia
/
Triptófano
/
Técnicas In Vitro
/
Química
/
Vellosidades Coriónicas
/
Medios de Cultivo Condicionados
/
Quinurenina
/
Metabolismo
Límite:
Femenino
/
Humanos
/
Embarazo
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2011
Tipo del documento:
Artículo
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