Interaction between Neuronal Depolarization and MK-801 in SH-SY5Y Cells and the Rat Cortex
Psychiatry Investigation
;
: 94-101, 2008.
Artículo
en Inglés
| WPRIM
| ID: wpr-33387
ABSTRACT
OBJECTIVE:
The interaction between MK-801, a model of psychosis and KCl-induced depolarization or electroconvulsive shock (ECS), a therapeutic model of electroconvulsive therapy (ECT), was investigated in SH-SY5Y cells and the rat frontal cortex.METHODS:
SH-SY5Y cells were pretreated with 1 microM MK-801 for 15 min, followed by cotreatment with 100 mM KCl for 5 min. MK-801 was reintroduced after the KCl was washed out, and the samples were incubated before harvesting. For the experiments in rats, male Sprague-Dawley rats were treated with MK-801 followed by ECS. Immunoblot analyses of glycogen synthase kinase 3beta (GSK3beta) (Ser9), AKT (Ser473) and extracellular legulated kinase (ERK)1/2 in SH-SY5Y cells and the rat frontal cortex were performed.RESULTS:
KCl-induced neuronal depolarization resulted in the transient dephosphorylation of AKT (Ser473) and GSK3beta (Ser9), followed by increased phosphorylation of the enzymes in SH-SY5Y cells. Cotreatment with MK-801 and KCl inhibited the initial dephosphorylation of AKT and GSK3beta produced by KCl-induced neuronal depolarization. Similarly, ECS resulted in the transient dephosphorylation of AKT (Ser473) and GSK3beta (Ser9), whereas cotreatment with MK-801 inhibited the initial dephosphorylation of AKT (Ser473) and GSK3beta (Ser9) produced by ECS in the rat frontal cortex. No significant interaction was observed between MK-801 and KCl in the dephosphorylation of ERK1/2.CONCLUSION:
These results suggest that an antagonistic interplay between MK-801 and neuronal depolarization by KCl or ECS is involved the regulation of AKT (Ser473) and GSK3beta (Ser9) phosphorylation.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fosforilación
/
Fosfotransferasas
/
Trastornos Psicóticos
/
Maleato de Dizocilpina
/
Ratas Sprague-Dawley
/
Glucógeno Sintasa Quinasas
/
Terapia Electroconvulsiva
/
Electrochoque
/
Neuronas
Límite:
Animales
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Psychiatry Investigation
Año:
2008
Tipo del documento:
Artículo
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