Differential expression of secretagogin and glucose-related protein 78 in colorectal carcinoma: a proteome study / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 107-112, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-333958
ABSTRACT
<p><b>OBJECTIVE</b>To identify the differentially expressed proteins or peptides and potential biomarkers of tumorigenesis for colorectal cancers.</p><p><b>METHODS</b>Immobilized pH gradient two-dimensional gel electrophoresis (2-DE) was used to separate and obtain the differentially expressed protein spots between colorectal cancers and matched normal mucosa. Liquid chromatography/mass spectrometry (LC-MS/MS) was used to characterize these proteins. Selected candidate proteins were further studied by Western blot, semi-quantitative RT-PCR and immunohistochemical staining.</p><p><b>RESULTS</b>Thirty-five protein spots showed marked expression changes (more than 5-fold) in colorectal carcinoma compared to normal mucosa. Fifteen proteins were up regulated and 20 were down regulated. Fourteen of these proteins were identified by tandem mass spectrometry, among which secretagogin (SCGN) was down-regulated and glucose-related protein (GRP) 78 was up-regulated in the tumors. The SCGN down-regulation was further supported by Western blot and RT-PCR analyses. Immunohistochemistry revealed that SCGN was strongly expressed in neuroendocrine cells of the colonic crypts and 53 of 54 (98%) neuroendocrine tumors. At protein level, although GRP78 was up regulated in colorectal carcinoma, there was no difference in the mRNA expression level between the tumor and paired normal mucosa.</p><p><b>CONCLUSIONS</b>The 2-DE combined with MS is a powerful tool for screening potential tumor biomarkers. The differentially expressed candidate proteins identified by 2-DE may be of significance in understanding the tumorigenesis of the colon cancer. SCGN is a potential biomarker for neuroendocrinal differentiation. GRP78 up-regulation in colorectal carcinomas may be related to its post-translational modification.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas de Unión al Calcio
/
ARN Mensajero
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Inmunohistoquímica
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Neoplasias Colorrectales
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Electroforesis en Gel Bidimensional
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Biomarcadores de Tumor
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Regulación Neoplásica de la Expresión Génica
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Tumores Neuroendocrinos
/
Chaperonas Moleculares
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Pathology
Año:
2007
Tipo del documento:
Artículo
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