Expression of Toll-like receptors in human bone marrow mesenchymal stem cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 695-699, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-334043
ABSTRACT
The aim of this study was to explore the characteristics of Toll-like receptor expression in mesenchymal stem cells derived from bone marrow of healthy donor (BM-MSCs). BM-MSCs were isolated from bone marrow of healthy donor by Ficoll method. Expressions of CD34, CD45, HLA-DR, CD44 and CD71 in BM-MSCs were detected by flow cytometry. CD71 in BM-MSCs was assayed by immunocytochemistry. The adipocyte and osteoblast induction of BM-MSCs were detected by alizarin red stain and oil red stain respectively. TLR 1 - 10 mRNA levels in BM-MSCs were evaluated by semiquantitative RT-PCR. The results showed that expressions of CD34, CD45 and HLA-DR in BM-MSC were negative while the expressions of CD44 and CD71 were positive. CD71 in BM-MSCs was positive. After induced by osteoblast and adipocyte inductor, BM-MSCs were positive for alizarin red staining and oil red staining respectively. All of TLR 1 - 10 mRNA were found in BM-MSCs with high expression levels of TLR2, TLR3, TLR4, TLR7, TLR8, TLR9 and low expression levels of TLR1, TLR5, TLR6, TLR10. In conclusion, different levels of TLR 1 - 10 mRNA were expressed in BM-MSCs of healthy donor.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ARN Mensajero
/
Células de la Médula Ósea
/
Diferenciación Celular
/
Células Cultivadas
/
Receptores Toll-Like
/
Células Madre Mesenquimatosas
/
Genética
/
Metabolismo
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2009
Tipo del documento:
Artículo
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