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Effect of RAR-beta transfection on the proliferation and phenotype of rat hepatic stellate cells / 中华肝脏病杂志
Chinese Journal of Hepatology ; (12): 297-300, 2002.
Artículo en Chino | WPRIM | ID: wpr-334220
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of RAR-beta transfection plus treatment with the corresponding ligand ATRA on the proliferation and phenotype of platelet-derived growth factor (PDGF)-activated hepatic stellate cells (HSC).</p><p><b>METHODS</b>PDGF-activated hepatic stellate cells of rats were transfected with eukaryotic expression vector pCMV-script-RAR-beta, which was verified by western blot. The proliferation of transfected HSC was assayed by BrdU incorporation as well as MTT methods. Their phenotype (alpha-SMA and desmin) was observed by immunocytochemistry assay with image analysis and RAR-beta protein expression was detected by western blot.</p><p><b>RESULTS</b>Transfection of RAR-beta gene and treatment with ligand ATRA could increase the expression of RAR-beta protein for at least 144h and inhibit the proliferation and the expression of alpha-SMA and desmin in PDGF-activated HSC. Significant statistical differences were perceived comparing with sham-transfected, only-PDGF treated, non-ligand treated and irrelevant ligand-treated HSC.</p><p><b>CONCLUSIONS</b>Transfected with RAR-beta gene as well as using related ligand ATRA could suppress the proliferation and reverse the activation phenotype of activated HSC.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Fenotipo / Fisiología / Tretinoina / Factor de Crecimiento Derivado de Plaquetas / Transfección / División Celular / Western Blotting / Receptores de Ácido Retinoico / Biología Celular Límite: Animales Idioma: Chino Revista: Chinese Journal of Hepatology Año: 2002 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Fenotipo / Fisiología / Tretinoina / Factor de Crecimiento Derivado de Plaquetas / Transfección / División Celular / Western Blotting / Receptores de Ácido Retinoico / Biología Celular Límite: Animales Idioma: Chino Revista: Chinese Journal of Hepatology Año: 2002 Tipo del documento: Artículo