Rapid induction of mRNAs for liver regeneration genes by hepatopoietin and partial hepatectomy / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 256-259, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-334235
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of recombinant human hepatopoietin (rhHPO) and partial hepatectomy on rapidly induced expression of immediate early gene.</p><p><b>METHODS</b>We investigated the different gene expression within 1 hour after 2/3 partial hepatectomy by representational difference analysis and in primary cultured hepatocytes system.</p><p><b>RESULTS</b>In the expressed sequence tag (EST) library, we identified that most of these genes were immediate early gene, and found one new gene PC3 that might be associated to liver regeneration in the EST library. Moreover, PC3 gene was rapidly induced after 2/3 partial hepatectomy and the expressing peak was within 1~2 hours after operation. HPO can rapidly induce the expression of these genes (c-fos, LRF-1, and PC3, etc.) in primarily cultured rat hepatocyte, which might be one of HPO molecular mechanism on stimulating hepatocyte proliferation.</p><p><b>CONCLUSIONS</b>rhHPO and partial hepatectomy can rapidly induce the expression of immediate early gene. PC3 gene is immediate early gene related to liver regeneration.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Proteínas Recombinantes
/
ARN Mensajero
/
Regulación de la Expresión Génica
/
Northern Blotting
/
Ácido Aspártico Endopeptidasas
/
Ratas Wistar
/
Factor de Crecimiento de Hepatocito
/
Genes Inmediatos-Precoces
/
Proproteína Convertasas
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Hepatology
Año:
2002
Tipo del documento:
Artículo
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