Establishment of RT- LAMP for rapid detection of foot-and-mouth disease virus / 病毒学报
Chinese Journal of Virology
;
(6): 137-142, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-334733
ABSTRACT
A rapid detection of foot-and-mouth disease virus (FMDV) was established by using reverse transcription loop-mediated isothermal amplification (RT-LAMP) method, meanwhile its specificity and sensitivity were assessed. The results showed that the FMDV RNA could be amplified by incubation at 65degrees C for only 1h using six primers designed based on FMDV polyprotein gene and the amplification products could be detected easily by naked-eye. There is no cross reaction with other virus such as SVDV, SFV and PPV by detecting their RNA samples. The detection limit of this method was found to be 10(-5) dilution of RNA sample which was 100-fold higher than that of PCR and 10-fold higher than real-time PCR.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Enfermedad Vesicular Porcina
/
Virología
/
ARN Viral
/
Sensibilidad y Especificidad
/
Genoma Viral
/
Cartilla de ADN
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Técnicas de Amplificación de Ácido Nucleico
/
Virus de la Fiebre Aftosa
/
Fiebre Aftosa
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2009
Tipo del documento:
Artículo
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