Construcion of a chimeric Japanese encephalits virus/dengue virus-2 / 病毒学报
Chinese Journal of Virology
;
(6): 185-189, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-334753
ABSTRACT
The prM/E gene of DV2 was cloned into the JEV (SA14-14-2 strain) replicon vector which had been constructed previously, and the resulting recombinant plasmid was named pPartialdeltaprM/E. The constructed chimeric clone was linearized and then was transcripted into RNA in vitro. The produced RNA was transfected into BHK-21 cells. Five to seven days later, CPE could be observed on the transfected BHK-21cells, and then the supernatant containing the chimeric virus was collected. The Supernatant was inoculated to BHK-1 cells and C6/36 cells, respectively. CPE could be observed about 4 days post the infection of C6/36cell with the chimeric virus. The results from RT-PCR, IFA, Western blot showed that the virus contained the chimeric RNA and the envelop protein of DV2. However, the chimeric virus could not be passaged in BHK-21 cell. The successful construction of the infectious clone JE/DEN-2 laid the basis for the further research of the DV vaccine.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Recombinación Genética
/
Línea Celular
/
Western Blotting
/
Virus Reordenados
/
Virus de la Encefalitis Japonesa (Subgrupo)
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Virus del Dengue
/
Vectores Genéticos
/
Genética
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2009
Tipo del documento:
Artículo
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