Cloning and expression of single-chain Fv antibodies against H5N1 Avian influenza virus hemagglutinin / 病毒学报
Chinese Journal of Virology
;
(6): 63-67, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-334761
ABSTRACT
To construct Fv antibodies against H5N1 Avian influenza virus hemagglutinin,extracted mRNA from B lymphoblastoid cell lines secreting anti-HA antibodies was used and the VH and VL genes were amplified by RT-PCR and linked together by splicing overlap extension (SOE) with (Gly4 Ser)3 linker. The recombinant plasmid was then transformed to E. coli BL21(DE3) and sequence analysis indicated the total length of scFv was 714 bp and the expression of Fv was validated by PAGE and Western blot.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Proteínas Virales
/
Virología
/
Aves
/
Proteínas Recombinantes de Fusión
/
Región Variable de Inmunoglobulina
/
Regulación de la Expresión Génica
/
Clonación Molecular
/
Cadenas Pesadas de Inmunoglobulina
/
Cadenas Ligeras de Inmunoglobulina
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2009
Tipo del documento:
Artículo
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