Cross reactivity of mimotopes of hepatitis C virus hypervariable region 1 / 病毒学报
Chinese Journal of Virology
;
(6): 249-254, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-334815
ABSTRACT
The cross reactivity of mimotopes of hepatitis C virus (HCV) hypervariable region 1 (HVR1) was investigated to obtain epitopes that have high cross reactivity. Five expression vectors encoding B cell mimotopes fused with Trx were constructed, and the mimotope proteins were purified. The cross reactivity of mimotope proteins with HCV positive sera was determined by ELISA. HCV pseudotype particles (HCVpp) were generated and applied to evaluate neutralization effects of the sera of BALB/c mice immuned with the mimotope proteins on infection of Huh7. 5 cells. Our data showed that the mimotope proteins (P1, P2, P5, P6, P8) could react to the HCV positive sera. The HCVpp infection inhibition of the sera of BALB/c mice immuned with P6 or P8 was detectable. These results suggest that the mimotopes may be valuable in the studies of anti-HCV infection and development of HCV vaccines.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
/
Datos de Secuencia Molecular
/
Secuencia de Aminoácidos
/
Hepacivirus
/
Reacciones Cruzadas
/
Regiones Determinantes de Complementariedad
/
Alergia e Inmunología
/
Ratones Endogámicos BALB C
/
Epítopos
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2008
Tipo del documento:
Artículo
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