Construction of recombinant fowlpox virus expressing E0 gene of classical swine fever virus shimen strain and the animal immunity experiment / 病毒学报
Chinese Journal of Virology
;
(6): 59-63, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-334848
ABSTRACT
The CSFV E0 gene was amplified from the plasmid pMD18-T-E0 by PCR and cloned into the FPV-P11 and FPV-pSY. The identified recombinant DNA was transfected into chicken embryo fibroblasts (CEF) to package Fowlpox virus. E0 gene was confirmed to be integrated into the genome of recombinant Fowlpox virus by PCR, and Western blot was employed for detection of E0 expression in the chicken embryo fibroblasts infected with recombinant Fowlpox virus . The results of ELISA showed that systemic immune response to CSFV could be induced effectively after the mice were immunized three times with recombinant Fowlpox virus through celiac route, the titer of antibody was 1 4096. The protection experiment showed that 75% of piglets immunized three times with recombinant Fowlpox virus were survived, indicating that the recombinant Fowlpox virus was effective. This paper lays foundation for the study of CSFV live vector vaccine.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Porcinos
/
Ensayo de Inmunoadsorción Enzimática
/
Vacunas Virales
/
Vacunas Sintéticas
/
Proteínas del Envoltorio Viral
/
Western Blotting
/
Reacción en Cadena de la Polimerasa
/
Alergia e Inmunología
/
Virus de la Viruela de las Aves de Corral
/
Genética
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2008
Tipo del documento:
Artículo
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