Sequence analysis for the complete provial genome of endogenous avian leukosis virus strain SD0501 / 病毒学报
Chinese Journal of Virology
;
(6): 53-58, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-334849
ABSTRACT
The genomic DNA extracted from chicken embryo fibroblasts (CEF) of SPF chickens from three chicken farms was used as template to amplify the ALV proviral DNA by PCR with four pairs of primers, high positive detection rates of gag - gene (29/46), pol - gene (27/46), env - gene (24/46) and LTR fragment (31/46) were achieved. Eight continuous and overlapping fragments were amplified from one DNA sample with 8 pairs of primers according to published sequences, then cloned into the TA vector and se quenced. The complete sequence of the whole genome of ALV strain SD0501 was established and analyzed with DNAstar software. Comparisons of SD0501 sequence with that of other representative endogenous avian virus strains demonstrated that the genomes of ALV were relatively conservative, the nucleotide identity of all the strains was over 99.1%, and env - gene was over 98.5%. However, a low identity was demonstrated among the representative strains of different subgroups, especially, the env - gene showed obvious difference, the corresponding identity was as low as 56.3% - 91.5%.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Secuencia de Bases
/
Reacción en Cadena de la Polimerasa
/
Provirus
/
Genoma Viral
/
Análisis de Secuencia de ADN
/
Organismos Libres de Patógenos Específicos
/
Virus de la Leucosis Aviar
/
Secuencias Repetidas Terminales
/
Genética
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2008
Tipo del documento:
Artículo
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