Construction and immunogenicity study of DNA vaccine expressing human H5N1 influenza virus hemagglutinin / 病毒学报
Chinese Journal of Virology
;
(6): 366-370, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-334881
ABSTRACT
Based on the first isolated human H5N1 influenza virus strain A/Anhui/1/2005 in China, HA and HA1 genes were amplified and cloned into the eukaryotic expression vector pStar. The recombinant plasmids pStar-HA and pStar-HA1 were transfected into COS7 cells. Western blot and IFA showed that the two recombinant DNA plasmids were successfully expressed in eukaryotic cells. BALB/c mice were immunized with the plasmids DNA by intramuscular injection. Anti-HA specific antibody in peripheral blood of immunized mice was tested by ELISA. The results showed that the recombinant plasmids successfully induced the anti-HA humoral immune response, and there was no significant difference between HA and HA1 as immunogen. This work provides basis for future development of novel avian flu vaccine.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sangre
/
Inmunoglobulina G
/
Vacunas contra la Influenza
/
Chlorocebus aethiops
/
Células COS
/
Vacunas de ADN
/
Glicoproteínas Hemaglutininas del Virus de la Influenza
/
Alergia e Inmunología
/
Subtipo H5N1 del Virus de la Influenza A
/
Genética
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2007
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS