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Construction and immunogenicity study of DNA vaccine expressing human H5N1 influenza virus hemagglutinin / 病毒学报
Chinese Journal of Virology ; (6): 366-370, 2007.
Artículo en Chino | WPRIM | ID: wpr-334881
ABSTRACT
Based on the first isolated human H5N1 influenza virus strain A/Anhui/1/2005 in China, HA and HA1 genes were amplified and cloned into the eukaryotic expression vector pStar. The recombinant plasmids pStar-HA and pStar-HA1 were transfected into COS7 cells. Western blot and IFA showed that the two recombinant DNA plasmids were successfully expressed in eukaryotic cells. BALB/c mice were immunized with the plasmids DNA by intramuscular injection. Anti-HA specific antibody in peripheral blood of immunized mice was tested by ELISA. The results showed that the recombinant plasmids successfully induced the anti-HA humoral immune response, and there was no significant difference between HA and HA1 as immunogen. This work provides basis for future development of novel avian flu vaccine.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Sangre / Inmunoglobulina G / Vacunas contra la Influenza / Chlorocebus aethiops / Células COS / Vacunas de ADN / Glicoproteínas Hemaglutininas del Virus de la Influenza / Alergia e Inmunología / Subtipo H5N1 del Virus de la Influenza A / Genética Límite: Animales Idioma: Chino Revista: Chinese Journal of Virology Año: 2007 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Sangre / Inmunoglobulina G / Vacunas contra la Influenza / Chlorocebus aethiops / Células COS / Vacunas de ADN / Glicoproteínas Hemaglutininas del Virus de la Influenza / Alergia e Inmunología / Subtipo H5N1 del Virus de la Influenza A / Genética Límite: Animales Idioma: Chino Revista: Chinese Journal of Virology Año: 2007 Tipo del documento: Artículo