Cloning NS1 gene of H5N1 avian influenza virus and apoptosis induced by it in human pulmonary carcinoma cell line A549 / 病毒学报
Chinese Journal of Virology
;
(6): 360-365, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-334882
ABSTRACT
The NS1 gene of the H5N1 subtype avian influenza virus was amplified by RT-PCR, and the am-plified product was cloned into the eukaryotic expression vector pCMV-Myc, then it was transfected into A549 cells. After 48 h, the expression of NS1 was detected by Western blot. Fluorescence and electron microscopy and flow cytometry showed that the NS1 gene of H5N1 avian influenza virus could induce apop-tosis in human pulmonary carcinoma cell line A549.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Fisiología
/
Virulencia
/
Clonación Molecular
/
Proteínas no Estructurales Virales
/
Apoptosis
/
Anexina A5
/
Línea Celular Tumoral
/
Subtipo H5N1 del Virus de la Influenza A
/
Genética
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2007
Tipo del documento:
Artículo
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