Construction and application of subgenomic replicon vectors of Japanese encephalitis virus / 病毒学报
Chinese Journal of Virology
;
(6): 33-38, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-334913
ABSTRACT
Based on the infectious clone of JEV vaccine SA14-14-2, the subgenomic replicons pCTCJEV, pCTMJEV with large deletions in the structural region were constructed. Then they were transfected into BHK-21 cell, the RNA replication of JEV subgenome can be monitored by RT-PCR and the non-structural protein can be found expressed in the cell by IFA. To explore the possibility of using a reporter gene assay to monitor synthesis of the positive-strand and the negative-strand JEV RNA, we inserted an enhanced green fluorescence protein (EGFP) gene into the 3'-UTR of pCTCJEV, pCTMJEV under the control of the internal ribosomal entry site (IRES) of encephalomyelocarditis virus RNA. After transfection, the EGFP fluorescence could be seen under the fluorescence microscope 1 day later,and maintained for more than a week with no apparent cytopathic effect. The constructed JEV replicons would provide valuable tools to provide a possible vector for a long-lasting RNA virus expression system.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Replicón
/
Factores de Tiempo
/
ARN Viral
/
Línea Celular
/
Regiones Promotoras Genéticas
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Proteínas Fluorescentes Verdes
/
Virus de la Encefalitis Japonesa (Especie)
/
Vectores Genéticos
/
Genética
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2007
Tipo del documento:
Artículo
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