Silencing HSV1 gD expression in cultured cells by RNA interference / 病毒学报
Chinese Journal of Virology
; (6): 22-27, 2007.
Article
en Zh
| WPRIM
| ID: wpr-334915
Biblioteca responsable:
WPRO
ABSTRACT
To explore the anti-HSV-1 effect of silencing gD gene expression by RNA interference, five 21-nucleotide duplex small interfering RNAs(siRNAs) targeting the HSV1 gD sequence were designed and the gD-EGFP fusion gene expression vector was constructed, then co-transfected into Vero cell, and screened the effective siRNA through analyzing the intensity of the EGFP fluorescence. Finally, the anti-HSV1 effect was confirmed by plaque reduction assay, real-time PCR and daughter virus titration of HSV1 infected Vero cells transfected with siRNAs. The study demonstrated that siRNAs could effectively and specifically inhibit gD gene expression in HSV1-infected cells, but only had a little effect on HSV1 infection, so taking gD as the target of siRNA against HSV1 needs further study.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Proteínas Recombinantes de Fusión
/
Células Vero
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Transfección
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Chlorocebus aethiops
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Proteínas del Envoltorio Viral
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Reacción en Cadena de la Polimerasa
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Herpesvirus Humano 1
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ARN Interferente Pequeño
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Interferencia de ARN
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Proteínas Fluorescentes Verdes
Límite:
Animals
/
Humans
Idioma:
Zh
Revista:
Chinese Journal of Virology
Año:
2007
Tipo del documento:
Article