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Silencing HSV1 gD expression in cultured cells by RNA interference / 病毒学报
Chinese Journal of Virology ; (6): 22-27, 2007.
Article en Zh | WPRIM | ID: wpr-334915
Biblioteca responsable: WPRO
ABSTRACT
To explore the anti-HSV-1 effect of silencing gD gene expression by RNA interference, five 21-nucleotide duplex small interfering RNAs(siRNAs) targeting the HSV1 gD sequence were designed and the gD-EGFP fusion gene expression vector was constructed, then co-transfected into Vero cell, and screened the effective siRNA through analyzing the intensity of the EGFP fluorescence. Finally, the anti-HSV1 effect was confirmed by plaque reduction assay, real-time PCR and daughter virus titration of HSV1 infected Vero cells transfected with siRNAs. The study demonstrated that siRNAs could effectively and specifically inhibit gD gene expression in HSV1-infected cells, but only had a little effect on HSV1 infection, so taking gD as the target of siRNA against HSV1 needs further study.
Asunto(s)
Texto completo: 1 Índice: WPRIM Asunto principal: Proteínas Recombinantes de Fusión / Células Vero / Transfección / Chlorocebus aethiops / Proteínas del Envoltorio Viral / Reacción en Cadena de la Polimerasa / Herpesvirus Humano 1 / ARN Interferente Pequeño / Interferencia de ARN / Proteínas Fluorescentes Verdes Límite: Animals / Humans Idioma: Zh Revista: Chinese Journal of Virology Año: 2007 Tipo del documento: Article
Texto completo: 1 Índice: WPRIM Asunto principal: Proteínas Recombinantes de Fusión / Células Vero / Transfección / Chlorocebus aethiops / Proteínas del Envoltorio Viral / Reacción en Cadena de la Polimerasa / Herpesvirus Humano 1 / ARN Interferente Pequeño / Interferencia de ARN / Proteínas Fluorescentes Verdes Límite: Animals / Humans Idioma: Zh Revista: Chinese Journal of Virology Año: 2007 Tipo del documento: Article