Effects of prenatal stress on kinetic properties of high-voltage-activated Ca2+ channel in freshly isolated offspring rat hippocampal CA3 pyramidal neurons / 南方医科大学学报
Journal of Southern Medical University
; (12): 1288-1292, 2006.
Article
en Zh
| WPRIM
| ID: wpr-334941
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of prenatal stress (PNS) on kinetic properties of high-voltage-activated (HVA) Ca(2+) channel in freshly isolated offspring rat hippocampal CA3 pyramidal neurons.</p><p><b>METHODS</b>The pregnant rats were exposed to restraint stress, and the hippocampal CA3 pyramidal neurons were freshly isolated from the offspring rats to record voltage-gate Ca(2+) channel currents in these neurons using whole-cell configuration of patch-clamp technique.</p><p><b>RESULTS</b>PNS significantly increased HVA calcium current density and integral current but did not alter the steady-state activation and steady-state inactivation properties of HVA Ca(2+) channel in the offspring rat CA3 neurons. The maximal HVA calcium current density was -40.89-/+0.31 pA/pF in the control group (n=10) and -49.44-/+0.37 pA/pF in PNS group (n=8, P<0.01). The maximal integral current of the HVA Ca(2+) channel was 106.81-/+4.20 nA*ms in the control group (n=10) and 133.49-/+2.59 nA*ms in the PNS group (n=8, P<0.01).</p><p><b>CONCLUSION</b>Maternal exposure to stress during the critical phase of pregnancy may result in long-lasting effects on the ion channels of the hippocampal neurons in the offspring rats.</p>
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Índice:
WPRIM
Asunto principal:
Fisiología
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Complicaciones del Embarazo
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Psicología
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Estrés Psicológico
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Restricción Física
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Cinética
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Canales de Calcio
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Ratas Sprague-Dawley
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Células Piramidales
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Técnicas de Placa-Clamp
Límite:
Animals
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Pregnancy
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2006
Tipo del documento:
Article