Labeling of CDTPA-dianhydride-coupled CD45 monoclonal antibody with yttrium-90 / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To explore the methods for labeling CDTPA-coupled CD45 monoclonal antibody (mAb) with yttrium-90 ((90)Y) for potential acute myeloid therapy.</p><p><b>METHODS</b>CD45 mAb was labeled with (90)Y by CDTPA and the labeling rate, radiochemical purity, final specific activity, and immunological activity of the mAb were detected.</p><p><b>RESULTS</b>With the optimal molar ratio of CDTPA/Ab at 201, the labeling rate was 95%, radiochemical purity 99.8%, and final specific activity 1.9 mCi/mg. This conjugate was stable in vitro with comparable immunological activity in comparison with unlabeled CD45 mAb.</p><p><b>CONCLUSION</b>(90)Y-CDTPA-CD45 mAb possesses good properties as an ideal targetting therapeutic agent for acute leukemia.</p>