Refolding and purification of recombinant human VEGF-121 expressed as inclusion bodies in Escherichia coli / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1083-1086, 2006.
Artículo
en Inglés
| WPRIM
| ID: wpr-334990
ABSTRACT
Vascular endothelial growth factor 121 (VEGF(121)) was expressed as inclusion bodies by recombinant Escherichia coli. High concentrations of both biomass (46 g dry cell/L) and VEGF(121) inclusion bodies (4.5 g/L) were obtained by applying a high-cell-density culture. After the inclusion bodies were washed and dissolved, VEGF(121) was refolded at 0.2 mg/ml by ultrafiltration in refolding buffer with a yield of 81%. Renatured VEGF(121) was purified by anion chromatography and Sephacry S-100 chromatography with purity higher than 95% and final purification yield of 31%. The purified VEGF(121) could stimulate the proliferation of human umbilical vein endothelial cells as demonstrated by a biological activity assay.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Proteínas Recombinantes
/
Línea Celular
/
Cuerpos de Inclusión
/
Química
/
Pliegue de Proteína
/
Biología Celular
/
Células Endoteliales
/
Factor A de Crecimiento Endotelial Vascular
/
Proliferación Celular
Límite:
Humanos
Idioma:
Inglés
Revista:
Journal of Southern Medical University
Año:
2006
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS