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the effects of insulin-like growth factor i (igf-i) on expression of vascular endothelial growth factor (vegf) mrna in mg-63 osteoblastlike cells
Journal of the Korean Association of Oral and Maxillofacial Surgeons ; : 363-369, 2005.
Artículo en Coreano | WPRIM | ID: wpr-33524
ABSTRACT

PURPOSE:

To determine the role of Insulin-like Growth Factor-I (IGF-I) in the regulation of Vascular Endothelial Growth Factor (VEGF) expression in MG-63 cells and then to find the mechanism b which this regulation occurs. MATERIALS AND

METHODS:

MG-63 cells were grown to confluence in 60-mm dishes. To determine the effects of IGF-I on expression of VEGF mRNA according to time and concentration, the cells were treated with 10 nM IGF-I, following isolation of total RNA and Northern blot analysis after 1, 2, 4, 8, 12, 24 hours and after 2 hours of treatment with 0.5, 2, 10, 25, 50 nM IGF-I respectively, isolation of total RNA and Northern blot analysis were followed. To determine the mechanism of action of IGF-I, inhibitors such as hydroxyurea (76.1 microgram/ml), actinomycin D (2.5 microgra/ml), cycloheximide (10 microgram/ml) were added 1 hour after treatment of 10 nM IGF-I.

RESULTS:

1. the expression of VEGF mRNA was increased with treatment of IGF-I. 2. The expression of VEGF mRNA was increased according to time- and concentration dependent manner of IGF-I. 3. The effect of IGF-I was decreased by hydroxyuera, actinomycin D, but not by cycloheximide.

CONCLUSION:

IGF-I regulate the expression of VEGF mRNA in the level of DNA synthesis and transcription. These results could suggest that IGF-I plays an important role in angiogenesis in the process of new bone formation and remodeling.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Osteogénesis / ADN / ARN / ARN Mensajero / Factor I del Crecimiento Similar a la Insulina / Northern Blotting / Cicloheximida / Dactinomicina / Factor A de Crecimiento Endotelial Vascular / Hidroxiurea Idioma: Coreano Revista: Journal of the Korean Association of Oral and Maxillofacial Surgeons Año: 2005 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Osteogénesis / ADN / ARN / ARN Mensajero / Factor I del Crecimiento Similar a la Insulina / Northern Blotting / Cicloheximida / Dactinomicina / Factor A de Crecimiento Endotelial Vascular / Hidroxiurea Idioma: Coreano Revista: Journal of the Korean Association of Oral and Maxillofacial Surgeons Año: 2005 Tipo del documento: Artículo