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Involvement of inhibition of nucleus GAPDH over-expression in erythropoietin's reduction of neuronal apoptosis induced by brain ischemia/reperfusion in rats / 生理学报
Acta Physiologica Sinica ; (6): 269-274, 2012.
Artículo en Chino | WPRIM | ID: wpr-335914
ABSTRACT
To study whether recombinant human erythropoietin (rhEPO) reduces neuronal apoptosis through inhibiting over-expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in nucleus induced by brain ischemia/reperfusion in rats, 48 adult Sprague-Dawley rats were randomly divided into 3 groups sham, saline and EPO groups. Animal models of brain ischemia/reperfusion were established by middle cerebral artery occlusion in rats. The effects of EPO on the sizes of ischemia tissue were observed by TTC staining. The over-expression of GAPDH in nucleus was detected by Hoechst-33258 and anti-GAPDH antibody double staining. The neuronal apoptosis in penumbral was detected by Nissl's staining and Hoechst-33258 immunofluorescence, respectively. The results showed that rhEPO treatment (3 000 U/kg, three times daily, i.p.) apparently reduced the sizes of infarct brain tissue in ischemia/reperfusion rats. rhEPO inhibited over-expression of GAPDH in nucleus of apoptotic neurons. In the meantime rhEPO decreased the number of apoptotic neurons in ischemia/reperfusion rats. These results suggest that rhEPO may induced reduction of neuronal apoptosis in penumbra may be through inhibiting over-expression of GAPDH in nucleus of apoptotic neurons induced by ischemia/reperfusion. Reduction of GAPDH over-expression in nucleus may play a pivotal role in EPO inhibiting neuronal apoptosis in cerebral ischemia/reperfusion rats, providing experimental evidence for EPO neuro-protecting effects against ischemia/reperfusion.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Farmacología / Encéfalo / Proteínas Recombinantes / Daño por Reperfusión / Isquemia Encefálica / Eritropoyetina / Ratas Sprague-Dawley / Apoptosis / Gliceraldehído-3-Fosfato Deshidrogenasa (Fosforilante) Límite: Animales / Humanos Idioma: Chino Revista: Acta Physiologica Sinica Año: 2012 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Farmacología / Encéfalo / Proteínas Recombinantes / Daño por Reperfusión / Isquemia Encefálica / Eritropoyetina / Ratas Sprague-Dawley / Apoptosis / Gliceraldehído-3-Fosfato Deshidrogenasa (Fosforilante) Límite: Animales / Humanos Idioma: Chino Revista: Acta Physiologica Sinica Año: 2012 Tipo del documento: Artículo