Multiplex fluorescent quantitative PCR for detecting deep fungal infection in patients with systemic lupus erythematosus / 南方医科大学学报
Journal of Southern Medical University
;
(12): 2112-2121, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-336008
ABSTRACT
<p><b>OBJECTIVE</b>To establish a rapid, sensitive and specific method based on multiplex fluorescent quantitative PCR for detection of deep infections with Candida albicans and Aspergillus flavus in patients with systemic lupus erythematosus (SLE).</p><p><b>METHODS</b>Two pairs of primers and Taqman probes were designed according to the gene sequences of Candida albicans and Aspergillus flavus available in American Type Culture Collection. The positivity rate, sensitivity and specificity of the multiplex fluorescent quantitative PCR-based method for detecting the fungal infection was tested in 20 specimens from SLE patients with Candida albicans and Aspergillus flavus infections, 20 specimens from SLE patients with suspected deep fungal infections, and 20 microbial samples other than Candida albicans or Aspergillus flavus.</p><p><b>RESULTS</b>The multiple fluorescence quantitative PCR-based method showed a positivity rate and specificity of both 100% for detecting Candida albicans and Aspergillus flavus infections in the SLE patients. This method resulted in a detection sensitivity of 75%, significantly higher than that of fugal culture method (40%, P<0.05).</p><p><b>CONCLUSIONS</b>The multiplex fluorescent real-time PCR-based method allows rapid, quantitative and simultaneous detection of deep Candida albicans and Aspergillus flavus infections with high sensitivity and specificity in SLE patients.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Aspergilosis
/
Aspergillus
/
Candida albicans
/
Candidiasis
/
China
/
Reacción en Cadena de la Polimerasa
/
Epidemiología
/
Sensibilidad y Especificidad
/
Diagnóstico
/
Fluorescencia
Tipo de estudio:
Estudio diagnóstico
Límite:
Adulto
/
Femenino
/
Humanos
/
Masculino
País/Región como asunto:
Asia
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2009
Tipo del documento:
Artículo
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