MTT assay for detecting osteosarcoma cell apoptosis / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1899-1901, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-336055
ABSTRACT
<p><b>OBJECTIVE</b>To assess the feasibility of using MTT assay for detecting the apoptosis of osteosarcoma cells following chemotherapy.</p><p><b>METHODS</b>The osteosarcoma cells derived form surgical specimens were cultured in RPMI 1640 medium supplemented with 10% calf serum. Chemotherapeutic agents were administered in the cell culture, and MTT assay was used to observe the cell apoptosis under optical microscope.</p><p><b>RESULTS</b>MTT staining accurately identified apoptosis of the osteosarcoma cells, and the apoptotic cells were easily distinguished from normal cells and dead cells.</p><p><b>CONCLUSION</b>MTT staining is convenient and practical for detecting osteosarcoma cell apoptosis.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Cirugía General
/
Sales de Tetrazolio
/
Tiazoles
/
Neoplasias Óseas
/
Células Tumorales Cultivadas
/
Protocolos de Quimioterapia Combinada Antineoplásica
/
Osteosarcoma
/
Apoptosis
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2009
Tipo del documento:
Artículo
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