Isolation, purification and functional identification of Syrian golden hamster islets / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1809-1811, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-336079
ABSTRACT
<p><b>OBJECTIVE</b>To establish the methods for isolation, purification and function identification of Syrian golden hamster islets.</p><p><b>METHODS</b>The Langerhans islets were isolated and purified from golden hamster pancreas by intra-ductal collagenase V perfusion and discontinuous Ficoll density gradient centrifugation. After isolation, the islet yield and purity were evaluated with DTZ staining. The islet function was assessed using glucose-stimulated insulin secretion test.</p><p><b>RESULTS</b>The total number of purified islets from one donor hamster was 359-/+35 islet equivalent (IEQ), with the purity and viability of the isolated islets of more than 90%. In response to glucose stimulations at 5.8 and 16.7 mmol/L, the secretion of insulin by the islets was 3.29-/+0.3 and 11.12-/+0.57 mU/L, respectively, showing a 2.28-fold higher insulin release by high-concentration than by low- concentration glucose stimulation (P<0.01).</p><p><b>CONCLUSION</b>The methods of collagenase V digestion and gradient centrifugation result in high yield and high purity of the isolated hamster islets.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Separación Celular
/
Islotes Pancreáticos
/
Mesocricetus
/
Secreciones Corporales
/
Biología Celular
/
Insulina
/
Métodos
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2009
Tipo del documento:
Artículo
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