Expression of porcine interferon-gamma and its safe antiviral assay / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 439-447, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-336208
ABSTRACT
In order to ensure the biosafety of the IFN-gamma antiviral activity assay, we used a replication-deficient VSV carrying GFP as an interferon sensitive indicator virus (VSVdeltaG*G). The antiviral activities of porcine IFN-gamma expressed in Escherichia coli and in baculovirus on MDBK cells were assessed. The results showed that the antiviral activity of porcine IFN-gamma expressed in baculovirus could reach 10(5) IU/mL, while the porcine IFN-gamma expressed in E. coli showed some antiviral activity (32 IU/mL) after refolding. The results of the VSVdeltaG*G-based antiviral assay were almost identical to that of the VSV*GFP-based assay, suggesting it is highly feasible to use VSVdeltaG*G as a substitute for VSV*GFP, making assays for IFN-gamma antiviral activity safer and more accurate.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Antivirales
/
Farmacología
/
Fisiología
/
Porcinos
/
Proteínas Recombinantes
/
Baculoviridae
/
Interferón gamma
/
Vesiculovirus
/
Proteínas Fluorescentes Verdes
/
Escherichia coli
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2010
Tipo del documento:
Artículo
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