Isolation and identification of Streptococcus mutans strains with different genotype from clinical samples / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 80-85, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-336387
ABSTRACT
<p><b>OBJECTIVE</b>To identify Streptococcus mutans (S. mutans) strains from clinical samples.</p><p><b>METHODS</b>Plaque samples from caries-active and caries-free sites on enamel surfaces were obtained and cultivated for S. mutans isolation. Morphology, biochemistry, automatic microorganism analysis system and polymerase chain reaction using primers homologous to surface protein antigen I/II (spaP), glucosyltransferase B (gtfB) and dextranase (dexA) were used to identify S. mutans. Genotype of isolated S. mutans was determined by arbitrarily primed polymerase chain reaction.</p><p><b>RESULTS</b>Forty-six strains of S. mutans were obtained from the 32 subjects and were identified as S. mutans by biochemistry, automatic microorganism analysis system and polymerase chain reaction. Five identical genotypes were found by arbitrarily primed polymerase chain reaction.</p><p><b>CONCLUSION</b>Forty-one strains of S. mutans with different genotype were obtained from clinical samples.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Streptococcus mutans
/
Reacción en Cadena de la Polimerasa
/
Caries Dental
/
Placa Dental
/
Genotipo
/
Glucosiltransferasas
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
Idioma:
Chino
Revista:
West China Journal of Stomatology
Año:
2013
Tipo del documento:
Artículo
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