Determination of nine penicillin residues in milk by high-performance liquid chromatography-mass spectrometry / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 171-177, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-336814
ABSTRACT
<p><b>OBJECTIVE</b>To establish a high-performance liquid chromatography-mass spectrometry (HPLC-MS/MS) method for detection of nine penicillin residues in milk.</p><p><b>METHODS</b>The samples were precipitated with acetonitrile and degreased with n-hexane for pre-processing. The chromatography was performed by reversed-phase HPLC in a C₁₈ column and eluted gradiently on line for 11 min. The mobile phases were water with formic acid (pH 3.1) and acetonitrile-water with formic acid (pH 3.1). By optimizing the mass condition, the method was operated by multiple-reaction monitoring (MRM) and quantitated by external standard calibration.</p><p><b>RESULTS</b>The standard curves were linear in concentration range of 0.4 approximately 400 μg/L and all nine penicillins showed a good linear relationship in the milk matrix (r>0.990). The detection limit of the method was from 0.1 to 0.8 μg/L while the limit of quantification was from 0.3 to 2.6 μg/L. The average recoveries were higher than 80%. The within-day precisions were less than 8.5%.</p><p><b>CONCLUSION</b>The established method is convenient, rapid and accurate and meets the requirement of monitoring penicillin residues in milk.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Penicilinas
/
Residuos de Medicamentos
/
Química
/
Cromatografía Líquida de Alta Presión
/
Leche
/
Espectrometría de Masas en Tándem
/
Métodos
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Zhejiang University. Medical sciences
Año:
2012
Tipo del documento:
Artículo
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