GLP-1 receptor activation effects the p38MAPK signal pathway in hepatic stellate cells / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effects of activation of the GLP-1 receptor on the p38MAPK signaling pathway in hepatic stellate cells (HSCs).</p><p><b>METHODS</b>HSCs were isolated and identified according to morphological features; the levels of GLP-1R protein were determined by western blotting.The HSCs were randomly divided into a control grouP (normal saline treatment) and experimental grouP(liraglutide treatment); after 120 hours, the expression of p38MAPK mRNA was examined by RT-PCR and of phosphorylated (p)-p38MAPK protein was detected by western blotting.</p><p><b>RESULTS</b>GLP-1R proteins were detected in the HSCs. Compared with the control group, the experimental group showed significantly decreased p38MAPK mRNA and p-p38MAPK protein (both P < 0.01).</p><p><b>CONCLUSION</b>The p38MAPK signaling pathway could be down-regulated when GLP-1R is activated in HSCs.</p>