Your browser doesn't support javascript.
loading
Preparation of anti-mouse PGRP-L single-epitope polyclonal antibody / 南方医科大学学报
Journal of Southern Medical University ; (12): 859-862, 2007.
Artículo en Chino | WPRIM | ID: wpr-337368
ABSTRACT
<p><b>OBJECTIVE</b>To prepare a single-epitope polyclonal antibody against mouse long peptidoglycan recognition protein (mPGRP-L).</p><p><b>METHODS</b>B cell dominant epitopes of mPGRP-L predicted by bioinformatics were synthesized, and the immunogen was prepared by conjugation of the synthetic peptide and the carrier protein key-hole limpet hemocyanin (KLH) by MBS method. The single-epitope polyclonal antibody was obtained by immunizing rabbits with the KLH-peptide conjugate, purified by SPG affinity columns or antigenic peptide affinity columns, and identified by ELISA and Western blotting.</p><p><b>RESULTS AND CONCLUSION</b>A dominant epitope in N-terminal region of mPGRP-L, with amino acid sequence of NH(2)-(C)DPHSLSPELQALISEVAQHD-COOH, was chosen and synthesized. The titer of anti-serum of the rabbits immunized with the KLH-peptide conjugate was 1256,000. The polyclonal antibody purified with SPG affinity columns and antigenic peptide affinity columns were named as mPGRP-Ln1 and mPGRP-Ln2, respectively. Western blotting demonstrated that the antibody mPGRP-Ln1 could recognize the recombined N-terminal fragment of mPGRP-Ln with a clear band at relative molecular mass of about 29,000, suggesting the successful preparation of the single-epitope polyclonal antibody against the N-terminal region of mPGRP-L.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Unión Proteica / Hemocianinas / Ensayo de Inmunoadsorción Enzimática / Datos de Secuencia Molecular / Proteínas Portadoras / Química / Western Blotting / Secuencia de Aminoácidos / Mapeo Epitopo / Alergia e Inmunología Límite: Animales Idioma: Chino Revista: Journal of Southern Medical University Año: 2007 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Unión Proteica / Hemocianinas / Ensayo de Inmunoadsorción Enzimática / Datos de Secuencia Molecular / Proteínas Portadoras / Química / Western Blotting / Secuencia de Aminoácidos / Mapeo Epitopo / Alergia e Inmunología Límite: Animales Idioma: Chino Revista: Journal of Southern Medical University Año: 2007 Tipo del documento: Artículo