Application of optimized multi-enzyme combination and sample pretreatment in proteomics / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 306-316, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-337413
ABSTRACT
Proteomics is a powerful subject focusing on large-scale study of protein structures and functions. A complete enzymatic digestion of protein complexes is the key step in modern high-resolution and high-throughput mass spectrometry (MS)-based identification and quantification. To achieve MS analysis, both peptide sample pretreatment and data acquisition are prerequisite in proteomic studies. In this paper, we summarized both the enzymatic proprieties of three common proteolytic enzymes, Trypsin, Lys-C and Glu-C, the optimization of multi-enzyme combination and an advanced sample pretreatment in proteomics research.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Espectrometría de Masas
/
Tripsina
/
Proteínas
/
Química
/
Proteómica
/
Enzimas
/
Métodos
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2016
Tipo del documento:
Artículo
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