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One-step fermentation for producing simvastatin via RNAi silencing of lovF gene in Aspergillus terreus / 生物工程学报
Chinese Journal of Biotechnology ; (12): 478-486, 2016.
Artículo en Chino | WPRIM | ID: wpr-337449
ABSTRACT
Simvastatin is one of the important prescription drugs for hypercholesterolemia. Monacolin J is a key intermediate during simvastatin synthesis, and also an intermediate of lovastatin biosynthesis. In this work, we construct a monacolin J producing strain via RNA interference to achieve one-step fermentation to obtain simvastatin. The lovF gene silencing plasmid pMHJ137 was constructed and transformed into Aspergillus terreus by Agrobacterium tumefaciens mediated transformation method. Precursor DMB-S-MMP was supplied during the fermentation to screen positive strains of transformants; which also further confirmed the simvastatin producing capability of MJ1-24 by one-step fermentation. Strain MJ1-24 produced monacolin J rather than lovastatin, and the feeding of DMB-S-MMP resulted in the generation of simvastatin. This study suggested that RNAi can efficiently silence the lovF gene of A. terreus and promote the simvastatin production by one-step fermentation.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Aspergillus / Microbiología Industrial / Química / Simvastatina / Interferencia de ARN / Fermentación / Genética / Ácido 3-Mercaptopropiónico / Naftalenos Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2016 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Aspergillus / Microbiología Industrial / Química / Simvastatina / Interferencia de ARN / Fermentación / Genética / Ácido 3-Mercaptopropiónico / Naftalenos Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2016 Tipo del documento: Artículo