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Automatically feeding strategy for 1,3-propanediol fermentation of Klebsiella pneumoniae LDH526 / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1520-1527, 2015.
Article en Zh | WPRIM | ID: wpr-337469
Biblioteca responsable: WPRO
ABSTRACT
1,3-propanediol is an important chemical used as building block for the synthesis of highly promising polyesters such as polytrimethylene terephthalate. A genetically modified Klebsiella pneumoniae LDH526 can use glycerol as sole carbon source and produce 1,3-propanediol with the titer above 90 g/L. A key factor affecting the production of 1,3-propanediol by the mutant K. pneumoniae is the accurate control of the feeding of glycerol. To generate a robust and reproducible fermentation process of 1,3-propanediol, we designed and optimized an automatically feeding strategy of glycerol based on fermentation kinetics. By coupling the substrate feeding rate with easily observed variables -pH and fermentation time, we have achieved self-starting glycerol feeding and dynamic control of the glycerol concentration during the fermentation process. This automated system allowed us to generate a reproducible, consistent and operator-independent process from lab-scale to production scale. The final concentration of 1,3-propanediol was above 95 g/L after 72 h.
Asunto(s)
Texto completo: 1 Índice: WPRIM Asunto principal: Glicoles de Propileno / Microbiología Industrial / Propilenglicol / Medios de Cultivo / Fermentación / Glicerol / Klebsiella pneumoniae / Metabolismo / Métodos Idioma: Zh Revista: Chinese Journal of Biotechnology Año: 2015 Tipo del documento: Article
Texto completo: 1 Índice: WPRIM Asunto principal: Glicoles de Propileno / Microbiología Industrial / Propilenglicol / Medios de Cultivo / Fermentación / Glicerol / Klebsiella pneumoniae / Metabolismo / Métodos Idioma: Zh Revista: Chinese Journal of Biotechnology Año: 2015 Tipo del documento: Article