Influence of Hypoxic Condition on Invasion of Cultured Trophoblast / 대한주산의학회잡지
Korean Journal of Perinatology
;
: 498-507, 1999.
Artículo
en Coreano
| WPRIM
| ID: wpr-33770
ABSTRACT
OBJECTIVE:
To investigated whether lowering oxygen tension affects invasion of cultured trophoblast.METHODS:
Trophoblasts were isolated from the normal placenta in early pregnancy(6-10 weeks in gestation). Isolated trophoblasts were cultured under normoxic(5% CO2, 95% humid air in incubator) and hypoxic(MERCK, 1% O2, 99% CO2) conditions for 24, 48 and 72 hours, respectively. The proliferation ability was measured using [H3] thymidine assay. Total RNA was extracted from the cultured trophoblasts. The expressions of matrix metalloproteinase(MMP-2) and tissue inhibitor of metallo- proteinase(TIMP-2) were determined by reverse transcription- polymerase chain reaction(RT-PCR) and Northern blot analysis. The invasiveness of cultured trophoblast was observed using in vitro invasion assay.RESULTS:
[H] thymidine assay indicated that cellular DNA synthesis was not affected by the culture condition. The expression of MMP-2 mRNA was decreased at 24 hours and then progressively increased in the time-dependent manner in each culture condition. The expression of TIMP-2 was decreased in the time-dependent manner under hypoxic condition. In vitro invasion assay revealed that the cultured trophoblasts under hypoxic condition has more invasive ability than them under normoxic condition.CONCLUSION:
These data suggests that hypoxic condition may stimulates the invasion of trophoblast in the human placentation. And MMP-2 and TIMP-2 may be related to control their invasiveness under hypoxic condition.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oxígeno
/
Placenta
/
Placentación
/
Timidina
/
Trofoblastos
/
ADN
/
ARN
/
ARN Mensajero
/
Northern Blotting
/
Inhibidor Tisular de Metaloproteinasa-2
Límite:
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Perinatology
Año:
1999
Tipo del documento:
Artículo
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