Humanin protects neurons against apoptosis induced by Abeta31-35 through suppression of intrinsic pathway / 生理学报
Acta Physiologica Sinica
; (6): 93-100, 2010.
Article
en En
| WPRIM
| ID: wpr-337773
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WPRO
ABSTRACT
The present study aimed to investigate the effects of humanin (HN) on primary cortical neuronal apoptosis induced by Abeta31-35, and explore the potential mechanisms. Cultured cortical neurons were pretreated with different concentrations of HN (5, 10, 20 micromol/L) for different time period (0, 8 and 16 h) respectively, and then exposed to Abeta31-35 (25 micromol/L) for additional 24 h and the neuronal apoptosis was examined by morphological analysis, flow cytometric assays and TUNEL staining. Caspase activities were measured using a spectrophotometer. Bax expression was measured by Western blot. The results were as follows. (1) Pretreatment with HN (20 micromol/L) for 16 h significantly prevented Abeta31-35-induced apoptosis in cortical neurons; (2) HN significantly decreased Abeta31-35-induced elevation of caspase-3 and -9 activities; (3) HN suppressed Abeta31-35-induced translocation of Bax from the cytosol to mitochondria, but had no effect on overall Bax expression. In conclusions, HN attenuated Abeta31-35-induced cortical neuronal apoptosis by blocking intrinsic caspase-dependent apoptotic pathways.
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Asunto principal:
Patología
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Fragmentos de Péptidos
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Farmacología
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Células Cultivadas
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Corteza Cerebral
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Péptidos beta-Amiloides
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Ratas Sprague-Dawley
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Apoptosis
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Fármacos Neuroprotectores
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Biología Celular
Límite:
Animals
Idioma:
En
Revista:
Acta Physiologica Sinica
Año:
2010
Tipo del documento:
Article