Role of activator protein-1 in pathogenesis of silicosis: an in-vitro study / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 12-15, 2008.
Article
en Zh
| WPRIM
| ID: wpr-338905
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of activator protein-1 (AP-1) in the up-regulation expression of tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta1(TGF-beta(1)) in silica-stimulated macrophage cells (RAW264.7).</p><p><b>METHODS</b>RAW264.7 cells were treated with AP-1 inhibitor Curcumin. The expression of c-jun and c-fos in nuclear protein was detected by western blotting. The level of TNF-alpha and TGF-beta(1) protein in the cell supernatant was measured using enzyme-linked immunoadsorbent assay (ELISA). Meanwhile the expression of TNF-alpha and TGF-beta(1) mRNA was also monitored by reverse transcriptase-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>The nucleoprotein expression of c-jun and c-fos in 10 and 20 micromol/L Curcumin prevention group (1.150 +/- 0.020, 1.010 +/- 0.108, 80.430 +/- 0.023, 0.256 +/- 0.015) were lower than those in silica-stimulated group (1.550 +/- 0.029, 0.860 +/- 0.036) (P < 0.01). In 20 micromol/L Curcumin prevention group and silica stimulated group, the expression of TNF-alpha protein were 23.58 +/- 45.78 and 32.12 +/- 5.34, and the expression of TGF-beta(1) protein were 1582.18 +/- 437.52 and 55.60 +/- 5.51 (P < 0.05 =; the expression of TNF-alpha, TGF-beta(1) mRNA were 0.74 +/- 0.01, 0.22 +/- 0.04 and 2.27 +/- 0.33, 2.96 +/- 0.15 (P < 0.05 =.</p><p><b>CONCLUSION</b>The expression of TNF-alpha, TGF-beta(1) mRNA and proteins is associated with activation of AP-1 in silica-stimulated macrophage cells.</p>
Texto completo:
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Índice:
WPRIM
Asunto principal:
Farmacología
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ARN Mensajero
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Línea Celular
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Factor de Necrosis Tumoral alfa
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Proteínas Proto-Oncogénicas c-jun
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Proteínas Proto-Oncogénicas c-fos
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Dióxido de Silicio
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Factor de Transcripción AP-1
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Curcumina
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Factor de Crecimiento Transformador beta1
Tipo de estudio:
Etiology_studies
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Año:
2008
Tipo del documento:
Article