Construction of anti-lung tumor gene differentially expressed bank of wild mouse / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 594-599, 2007.
Article
en Zh
| WPRIM
| ID: wpr-338977
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct the anti-lung tumor gene differentially expressed bank of wild mouse and to explore the mechanisms of the TW wild mouse suppressing the occurring of lung tumor.</p><p><b>METHODS</b>Using suppression subtractive hybridization (SSH) technique, the differentially expressed genes between TAF1 mouse and A/wy mouse were selected out and the subtracted cDNA bank was constructed. 166 clones were performed DNA sequencing and then were assayed by blast programme.</p><p><b>RESULTS</b>Among the blast results of 166 differentially expressed clones, 87 known genes (mRNA or cDNA) were in homology with 134 clones and were divided into 7 classifications according to the biological role.14 DNA fragments were in homology with 32 clones, in which 20 clones were in homology with 9 mouse DNA sequences, 2 clones were in homology with one bacterial gene sequences and 3 clones were clone vector.</p><p><b>CONCLUSION</b>With SSH technique, the anti-lung tumor gene differentially expressed bank of wild mouse are successfully constructed.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
ARN Mensajero
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Células Tumorales Cultivadas
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Biblioteca de Genes
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ADN Complementario
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Análisis de Secuencia por Matrices de Oligonucleótidos
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Perfilación de la Expresión Génica
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Genética
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Neoplasias Pulmonares
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Ratones Endogámicos
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Año:
2007
Tipo del documento:
Article